Table 3 Average cDNA pool coverages by enzyme combination across 92 eukaryotes.
From: Eukaryotic transcriptomics in silico: Optimizing cDNA-AFLP efficiency
Enzyme Combination | Coverage ± SD | Min-Max Coverage | R2 | Regression Equation |
|---|---|---|---|---|
MseI & CviAII | 76.13 ± 10.51 | 42.07 - 91.97 | 0.94 | Nbp = 1849399*AF+294835 |
CviAII & CviQI | 72.55 ± 10.32 | 46.44 - 93.68 | 0.98 | Nbp = 1924913*AF+1067365 |
CviAII & TaqI | 69.56 ± 13.62 | 32.19 - 96.94 | 0.85 | Nbp = 2152800*AF+933639 |
MseI & CviQI | 66.63 ± 10.32 | 36.63 - 86.15 | 0.94 | Nbp = 2731950*AF-862614 |
CviAII & MaeII | 64.16 ± 13.93 | 33.34 - 91.88 | 0.91 | Nbp = 2309466*AF+1759142 |
MaeI & CviAII | 63.20 ± 11.42 | 21.81 - 85.13 | 0.90 | Nbp = 2306663*AF+3307692 |
MseI & TaqI | 62.69 ± 14.23 | 28.35 - 90.91 | 0.72 | Nbp = 2995858*AF+626737 |
HpaII & CviAII | 62.00 ± 18.32 | 9.45 - 93.30 | 0.94 | Nbp = 1890564*AF+3666024 |
TaqI & CviQI | 61.26 ± 14.41 | 25.91 - 94.55 | 0.76 | Nbp = 2726475*AF+2079328 |
MseI & MaeI | 58.28 ± 12.22 | 27.61 - 79.49 | 0.84 | Nbp = 2852998*AF+2622539 |
MseI & MaeII | 57.15 ± 12.86 | 29.81 - 84.18 | 0.85 | Nbp = 3630319*AF-565133 |
MaeII & CviQI | 55.70 ± 15.43 | 23.57 - 88.18 | 0.85 | Nbp = 3156237*AF+1906975 |
MaeI & CviQI | 54.91 ± 11.08 | 21.14 - 81.68 | 0.91 | Nbp = 3400108*AF+2063017 |
TaqI & MaeII | 54.86 ± 17.23 | 19.16 - 92.44 | 0.64 | Nbp = 2762584*AF+4754785 |
HpaII & MseI | 54.39 ± 14.73 | 12.13 - 87.81 | 0.92 | Nbp = 3607697*AF+484514 |
HpaII & CviQI | 54.14 ± 18.34 | 9.05 - 91.74 | 0.88 | Nbp = 2623528*AF+3657451 |
HinP1I & CviAII | 53.57 ± 19.96 | 5.08 - 95.05 | 0.85 | Nbp = 2260683*AF+4996193 |
HpaII & TaqI | 53.54 ± 19.47 | 8.04 - 93.28 | 0.72 | Nbp = 2433331*AF+5040020 |
MaeI & TaqI | 52.26 ± 13.47 | 17.71 - 85.30 | 0.80 | Nbp = 4183276*AF+518528 |
HpaII & MaeII | 49.93 ± 19.70 | 5.49 - 89.29 | 0.79 | Nbp = 2690086*AF+4940474 |
HinP1I & CviQI | 47.88 ± 19.79 | 5.27 - 89.77 | 0.79 | Nbp = 2909507*AF+5271739 |
MaeI & MaeII | 47.76 ± 12.04 | 15.32 - 78.81 | 0.93 | Nbp = 4923589*AF-278386 |
HinP1I & TaqI | 47.32 ± 20.70 | 4.26 - 91.43 | 0.63 | Nbp = 2600056*AF+7269281 |
HinP1I & MseI | 46.22 ± 15.07 | 6.86 - 87.60 | 0.84 | Nbp = 4211767*AF+2594577 |
HpaII & MaeI | 45.75 ± 15.11 | 4.16 - 80.64 | 0.92 | Nbp = 4048888*AF+3106105 |
HinP1I & HpaII | 45.05 ± 22.78 | 1.36 - 94.75 | 0.78 | Nbp = 2272295*AF+6925901 |
HinP1I & MaeII | 44.15 ± 20.69 | 4.08 - 89.75 | 0.71 | Nbp = 2937393*AF+6703632 |
HinP1I & MaeI | 39.00 ± 15.30 | 2.53 - 77.47 | 0.89 | Nbp = 5159080*AF+3260514 |