The D7 protein family of An. darlingi and An. Gambiae. (A) Clustal alignment. (B) Phylogram based on the alignment in (A). The numbers on the tree nodes represent the percent bootstrap support in 10,000 trials. The bar at the bottom indicates 20% amino acid divergence. The An. gambiae sequence names start with D7 followed by s or L for short and long forms; the number following s or L represents the order of the gene in the D7 chromosomal region, following its transcription direction. The An. darlingi sequences start with AD, followed by a number derived from the cluster number, as determined in Supplemental Table S1. For more details, see text.