A microarray analysis of sex- and gonad-biased gene expression in the zebrafish: Evidence for masculinization of the transcriptome

Table 4 Top 15 ranked testis-upregulated genes within male zebrafish

GenBank acc. #	GenBank reference mRNA sequence	GCOS fold	GC-RMA fold	PM fold	PMMM fold
NM_001082815	similar to septin 4 (sept4)	590	1162	47	161
NM_212833	zgc:56699	404	1501	40	252
BI709397	unknown. No significant BLAST hits.	254	1026	51	96
BI709397	unknown. No significant BLAST hits.	412	451	36	331
NM_199958	*outer dense fiber of sperm tail gene 3-like* ( *odf3l* )	162	787	71	97
NM_212806	cytochrome P450, family 17, subfamily A, polypeptide 1 (cyp17a1)	157	368	92	184
NM_131057	*vasa homolog* (germ line development)	287	558	40	145
NM_001100021	UPF0722 protein, C11orf88 homolog	146	541	73	101
XM_692188	similar to CG14551 CG14551-PA	302	280	46	208
NM_001002357	zgc: 92129	349	1785	69	61
NM_001118894	*synaptonemal complex protein 1* ( *sycp1* )	452	191	62	191
NM_001007397	zgc:101797	168	848	58	69
XM_001342700	similar to predicted protein (LOC100003104)	166	1010	42	81
XM_692362	wu:fj98c04	187	401	34	185
NM_001089414	hypothetical protein zgc:162591	203	337	47	95

Fifteen highest ranking testis-upregulated genes (of 3002 total), determined by the mean of all four fold change rank values for each of the absolute expression analyses. Basic annotation is represented by a top MegaBLAST hit for each GeneChip^® probe set sequence, obtained by a search of the GenBank reference mRNA database. Any supplementary functional annotation information is included if available. E-values for the above BLAST searches are all 0.0, except for sept4 (5 e-65) and cyp17a1 (1 e-123). Several of the probe sets listed here lack any information with respect to a described mRNA counterpart, and many correspond to hypothetical protein-coding transcripts. Three of the well-annotated transcripts (in bold text), appear to be reproduction-related.

ISSN: 1471-2164