A microarray analysis of sex- and gonad-biased gene expression in the zebrafish: Evidence for masculinization of the transcriptome

Table 6 Real-time PCR expression confirmation

Gene class	Gene Name/ GenBank acc. #	Rank	GCOS fold	GC-RMA fold	PM fold	PMMM fold	qPCR 95% CI
Ovary-upregulated	casp3a NM_131887	121	3.0	4.7	2.4	2.7	4.6 -15
Ovary-upregulated	zgc:92067 NM_001002377	187	2.4	6.1	2.5	2.7	13 - 57
Testis-upregulated	sept4 NM_001082815	1	590	1162	47	161	189 - 518
Testis-upregulated	zgc:92129 NM_001002357	10	349	1785	69	61	740 - 3665
Male- enriched	fx05c05.x1 BM571726	4	810	545	38	283	189 - 488
Female-enriched	wu:fd20g04 XM_001334198	1	458	2190	246	551	1982 - >9999
Female-enriched	wu:fd14c01 XM_677844	2	572	1458	174	479	609 - 3246

Expression levels of sex- and gonad-biased zebrafish genes, as confirmed by quantitative real-time PCR. Included are each gene's expression bias category, GenBank identifier and accession number, within-category expression rank, four microarray fold change estimates based on different absolute expression analyses, and qPCR 95% confidence interval for fold change. Some of the confidence intervals are quite wide, but in every case statistically significant (p < 0.05) expression bias was confirmed.

ISSN: 1471-2164