Figure 6
From: Temperature Switch PCR (TSP): Robust assay design for reliable amplification and genotyping of SNPs
The effect of constant annealing temperature thermal cycling on TSP genotyping efficiency. Reactions were performed with an annealing temperature of 58°C for 35 cycles using 0.1 μM of LS primers (forward 5'-CGAGGATTGGCTCAAGACGC and reverse 5'-GCAGCGTTCTTAGGACTGGCA) and 0.5 μM of NLS primer (forward 5'-GGC CAGAGTAAGTTGCTGAA and reverse 5'-CGG TTGATCCCGTAGGTG). Underlined sequence indicates the non-complementary 5'-tail region. M represents a pUC19/HpaII DNA size ladder.