miRNAs
1
|
Gut_SF
6
|
Gut_BF
6
|
Fold Change I
7, 9
|
Fold Change II
8, 9
|
---|
aae-miR-11
2
|
42
|
436
|
6.55
|
8.16
|
aae-miR-1175*
|
2
|
59
|
18.61
|
23.18
|
aae-miR-13b
3
|
2
|
42
|
13.25
|
16.50
|
aae-miR-184
|
76
|
1307
|
10.85
|
13.51
|
aae-miR-190
|
2
|
43
|
13.56
|
16.89
|
aae-miR-281
|
16
|
50
|
1.97
|
2.46
|
aae-miR-281*
|
5686
|
4806
|
0.53
|
0.66
|
aae-miR-283
|
1
|
131
|
82.65
|
102.93
|
aae-miR-2a/2b/2c
3, 4
|
80
|
249
|
1.96
|
2.45
|
aae-miR-306
5
|
0
|
27
|
NA
10
|
NA
10
|
aae-miR-317-1
|
38
|
737
|
12.24
|
15.24
|
aae-miR-317-2
|
38
|
737
|
12.24
|
15.24
|
aae-miR-34
|
55
|
639
|
7.33
|
9.13
|
aae-miR-8
|
1
|
38
|
23.98
|
29.86
|
aae-miR-989
|
33
|
3
|
0.06
|
0.07
|
aae-miR-998
2
|
6
|
42
|
4.42
|
5.50
|
aae-miR-9b 5
|
0
|
69
|
NA
10
|
NA
10
|
- Notes.
- 1. Only miRNAs that showed 25 or more sequences in one of the gut samples are shown.
- 2. MiR-11 and miR-998 are physically linked, less than 300 bp apart.
- 3. MiR-13b and miR-2a/2b/2c are in a physically linked cluster. Mir-13b is less than 200 bp apart from miR-2b.
- 4. It is difficult to distinguish between hits that match miR-2a, miR-2b, or miR-2c. Thus we refer to these hits miR-2a/2b/2c.
- 5. MiR-306 and miR-9b are physically linked, less than 500 bp apart.
- 6. The second and third columns are raw numbers of sequence hits in sugar-fed (Gut_SF) and blood-fed (Gut_BF) midgut samples, respectively.
- 7. Column four is the fold difference of Gut_BF over Gut_SF, normalized by the total miRNA hits within a sample, as suggested in reference [12]. The total miRNA hits are 6260 and 9922 in Gut_SF and Gut_BF samples, respectively. Thus, for each miRNA, the Fold Change = [(Raw Gut_BF number)/9922]/[(Raw Gut_SF number)/6260].
- 8. Column five is the fold difference of Gut_BF over Gut_SF, normalized by the total small RNA reads within a sample. The total small RNA reads are 33, 000 and 42, 000 in Gut_SF and Gut_BF samples, respectively. Thus, for each miRNA, the Fold Change = [(Raw Gut_BF number)/42000]/[(Raw Gut_SF number)/33000].
- 9. The numbers in columns 4 and 5 reflect the same trend, generally higher levels of these miRNAs in blood-fed sample than in sugar-fed sample. Although the number of small RNA sequences may not be sufficient for the analysis to be quantitative, overall trends suggest leads for further analysis.
- 10. NA, not applicable as the denominator is zero.