DNA flexibility analysis of translocation-prone and fragile site co-localized genes. DNA sequences within and flanking genes (A) CBFB (B) MYH11 (C) HMGA1 (D) LAMA4 (E) MLL (F) AFF4 were analyzed using the FlexStab program. The analysis was performed over the length of the entire gene (shaded in black) plus 125 kb flanking on each side (shaded in gray). The x axis indicates the size of the analyzed sequences, and the y axis shows degrees of inclination in the twist angle. Windows with values > 13.7° were considered as significantly high flexibility peaks .