Skip to main content

Table 1 Array validation by Q-PCR

From: The iron/heme regulated genes of Haemophilus influenzae: comparative transcriptional profiling as a tool to define the species core modulon

  NTHi R2866 Hib 10810
Gene Arraya Q-PCRb Arraya Q-PCRb
HI0007 fdxI +7.49 +9.88 +6.00 +5.20
HI0017 yfiD -8.25 -10.72 -3.03 -3.34
HI0185 adhC +5.86 +7.65 +4.01 +6.52
HI0262 hxuC -24.69 -32.65 -4.03 -12.12
HI0502 rbsA ns +1.08 nsc +1.13
HI0591 potE -11.40 -13.39 -13.78 -2.91
HI0980 fis +4.67 +5.99 +2.56 +5.19
HI0994 tbp1 -9.56 -10.87 -8.16 -16.53
HI0997m ompU -15.68 -4.43 -14.61 -4.21
HI1349 dps -4.12 -3.03 -3.70 -4.81
HI1368 pqqL ns +1.67 ns -1.60
HI1384 ftnA1 +3.21 +4.34 +2.01 +4.43
HI1427 ABCt. -6.60 -5.18 -2.32 -2.64
HI1706 betT +3.24 +3.23 +3.39 +3.57
  1. aFold change between the FeHm unsupplemented and supplemented sample from the microarray.
  2. bFold change between the FeHm unsupplemented and supplemented sample from the Q-PCR analysis.
  3. cns, not significant