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Table 1 Array validation by Q-PCR

From: The iron/heme regulated genes of Haemophilus influenzae: comparative transcriptional profiling as a tool to define the species core modulon

 

NTHi R2866

Hib 10810

Gene

Arraya

Q-PCRb

Arraya

Q-PCRb

HI0007 fdxI

+7.49

+9.88

+6.00

+5.20

HI0017 yfiD

-8.25

-10.72

-3.03

-3.34

HI0185 adhC

+5.86

+7.65

+4.01

+6.52

HI0262 hxuC

-24.69

-32.65

-4.03

-12.12

HI0502 rbsA

ns

+1.08

nsc

+1.13

HI0591 potE

-11.40

-13.39

-13.78

-2.91

HI0980 fis

+4.67

+5.99

+2.56

+5.19

HI0994 tbp1

-9.56

-10.87

-8.16

-16.53

HI0997m ompU

-15.68

-4.43

-14.61

-4.21

HI1349 dps

-4.12

-3.03

-3.70

-4.81

HI1368 pqqL

ns

+1.67

ns

-1.60

HI1384 ftnA1

+3.21

+4.34

+2.01

+4.43

HI1427 ABCt.

-6.60

-5.18

-2.32

-2.64

HI1706 betT

+3.24

+3.23

+3.39

+3.57

  1. aFold change between the FeHm unsupplemented and supplemented sample from the microarray.
  2. bFold change between the FeHm unsupplemented and supplemented sample from the Q-PCR analysis.
  3. cns, not significant