Separation of proteins from H. armigera larval epidermis by 2-DE (2nd dimension: 12% SDS-PAGE). (A) 5th feeding (5th-F) larvae with Coomassie Brilliant Blue (CBB) stain. (B) 6th-metamorphically committed (6th-M) larvae with CBB staining. (C) 5th-F larvae with Pro-Q Diamond phosphoprotein gel (Pro-Q) stain. (D) 6th-M larvae with Pro-Q stain. Spots analyzed by mass spectrometry are designated by numbers. Spots annotated with the same number in both gels are the same spots visualized by different staining methods. The experiments were replicated three times.