Experimental evidences to explain transcriptional process in E. cuniculi. A: schematic representation of the genomic DNA region from the E. cuniculi chromosome 2 containing the genes unk, htr, h2a, pap, ubi and rib. White rectangles represent the position of the genes in genomic DNA (with gene names inside) and the arrowhead represents their transcriptional directions. The length in base pairs (bp) of the CDSs and the intergenic regions is also indicated. Black arrows represent the orientation of the specific primers used for RT, PCR and RACE-PCR experiments. B: results of RT-PCR experiments, indicating that both DNA strands are transcribed. Discontinuous lines indicate that respective transcription start site and transcription terminator one could not be defined. C: Schematic representation of RACE-PCR experiments. Black lines represent the length and position of the 5'-RACE (1) and the 3' RACE (2) fragments. PolyA tails are represented by AAAA and 5' end by a dot.