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Table 2 Study of Kozak sequences for correctly and mispredicted start codons.

From: Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation

POSITION

NUCLEOTIDE

correctly predicted AUG with CCC-like motif (1474)

mispredicted AUG (364)

corrected AUG and BLASTP validated (187)

corrected AUG but not BLASTP validated (177)

correctly predicted (117) and corrected (18) AUG with GGG-like motif

A

      

T

      

G

      

+4

A

37.65

21.15

41.71

36.16

48.15

 

T

10.92

22.53

10.16

11.86

7.41

 

G

40.57

30.49

41.714

42.94

34.81

 

C

10.85

25.82

6.42

9.04

9.63

+5

A

45.32

25.27

47.06

45.76

37.04

 

T

19.54

28.57

14.44

15.82

27.41

 

G

19.74

18.68

20.32

19.77

22.96

 

C

15.4

27.47

18.18

18.64

12.59

+6

A

26.87

24.73

28.88

27.12

32.59

 

T

23.47

32.14

20.86

23.16

23.7

 

G

28.7

22.53

26.2

30.51

28.15

 

C

20.96

20.6

24.06

19.21

15.56

  1. Nucleotide frequencies of occurrence downstream (+4 to +6) the ATG codons Significant nucleotide biases are indicated in bold