Figure 5

Comparison of expression of representative genes selected from microarray data with qRT-PCR. Comparison of fold change values from microarray data with expression ratios calculated from qRT-PCR. Values were determined using qRT-PCR and represents relative expression of genes between L5M and OHB. The relative expression of the target gene (Hb-sec-23: Yeast sec homolog, Hb-co-II: Cytochrome c oxidase II, Hb-dao-3: Dauer or aging adult overexpression, Hb-unc-68: Uncoordinated, Hb-asp-3: Aspartyl protease, Hb-hid-1: High temperature induced dauer formation, Hb-fat-2: Fatty acid desaturase, Hb-daf-21: Abnormal dauer formation, Hb-rab-33: RAB family member, Hb-spl-1: Sphingosine-1-phosphate lyase) normalized to Hb-18s:18S rRNA and relative to the expression of control. Bars represent standard errors calculated from 4 replicates of each experiment. *Significant difference (P < 0.05) between qRT-PCR and microarray data.