Figure 1
From: Exploiting proteomic data for genome annotation and gene model validation in Aspergillus niger
Schematic of the Average Peptide Scoring (APS) pipeline using reversed database searching. The final APS threshold is established iteratively for each pkl file searched against a given database, calculated over a range of peptide quality filters. Mascot was used to conduct an initial database search of both forward and reverse databases, and the resulting peptide scores were then used to calculate an average peptide score for matching proteins.