Different types of nucleosome occupancy change in the Plasmodium genome. Shown here are representatives of each type. The left panel was generated from the microarray data. Lines indicate average log2 ratio of H4 ChIP divided by genomic DNA hybridization intensity over 150 bp windows: blue = ring, green = trophozoite, red = schizont. Blue (Red) genes are encoded on the top (bottom) strand. The x-axis indicates the base pair position on the chromosome. The right panel shows Q-PCR results to validate the microarray results. The relative enrichment level represents the 2-ΔΔCt value obtained by comparing anti-H4 ChIP DNA with input DNA and normalizing the data with the control gene MAL8P1.36 which had relative stable nucleosome occupancy through IDC.