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Table 3 Differential metabolite pattern at pH 6 in comparison to pH 7.5

From: Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis

Metabolite differential content t-test
Methionine synthesis   
homolanthionine -15.28 0.0392
Serine -1.84 0.0307
Glycine -1.67 0.0010
homocysteine -1.41 0.2019
methionine -1.09 0.9449
threonine 1.26 0.0302
Aspartate 1.33 0.1295
homoserine 1.58 0.3969
O-acetyl-serine 2.34 0.0165
S-adenosyl-homocysteine 2.45 0.0441
cysteine 2.46 0.0030
O-acetyl-homoserine 3.71 0.0002
cystathionine 6.96 0.0044
Glycolysis and Pentosephosphate pathway
Pep -3.58 0.0025
DHAP -2.89 0.0004
DHAP -2.79 0.0010
glycerate-3-P -1.99 0.0043
gluconate-6-P -1.77 0.1733
ribose-5-P -1.44 0.0373
fructose-1-6-P 2.07 0.0360
glycerate-2-P 2.86 n.d.
fructose-6-P 3.00 0.0009
glucose-6-P 3.18 0.0000
glucose-6-P 3.85 0.0006
pyruvate 11.10 0.0341
TCA cycle
alpha-ketoglutarate -6.07 0.0131
succinate -5.37 0.0039
fumarate 1.25 0.1755
citrate 1.84 0.0775
malate 2.06 0.0065
Amino acids
proline -8.67 0.0006
beta-alanine -7.30 0.0000
ornithine, citrulline, arginine -2.38 0.2871
serine -1.84 0.0307
glycine -1.67 0.0010
lysine -1.58 0.3466
asparagine -1.45 0.3712
methionine -1.09 0.9449
leucine 1.01 0.9353
glutamate 1.02 0.7068
tyrosine 1.17 0.1464
threonine 1.26 0.0302
L-aspartate 1.33 0.1295
tryptophan 1.40 0.0606
isoleucine 1.73 0.0007
histidine 2.28 0.2898
cysteine 2.46 0.0030
alanine 3.28 0.0117
glutamine 4.85 0.0002
valine 5.52 0.0013
phenylalanine 9.08 0.0020
  1. Pool size ratios determined by GC-MS in extracts of C. glutamicum cells grown at pH 7.5 and pH 6. The ratios have been calculated using the mean values from two independently grown cultures and three technical replicates each. A t-test was applied for determining if observations were significantly different. Numbers in italics indicate values above the chosen significance cut off (P < 0.05), n.d. means not determined.