Table 1 Oligonucleotides for reverse-transcriptase PCR and sequencing reactions.
Name | Sequence | Target |
|---|---|---|
alpha1 | 5'-gtgactsacggccatgaggac-3' | putative α-defensin |
alpha2 | 5'-gtgactsacagccatgaggac-3' | putative α-defensin |
alpha3 | 5'-gtgactsatatccatgaggac-3' | putative α-defensin |
alpha4 | 5'-gtgactsatatacatgaggac-3' | putative α-defensin |
alpha5 | 5'-atgactcacagccatgaagac-3' | putative α-defensin |
alpha6 | 5'-gtgacccacagccatgaggac-3' | putative α-defensin |
alpha7 | 5'-gtgactgacagccataaggac-3' | putative α-defensin |
alpha2-1 | 5'-cctgacctccaggtgacccac-3' | putative α-defensin |
alpha2-2 | 5'-cctgacctccagttgactccc-3' | putative α-defensin |
alpha2-3 | 5'-attcacttccaggtgactcac-3' | putative α-defensin |
alpha2-4 | 5'-ctggaactccaggtgactcac-3' | putative α-defensin |
alpha2-5 | 5'-tctgaactccaggtgactcac-3' | putative α-defensin |
alpha2-6 | 5'-ggcctcagtcaggtgactgac-3' | putative α-defensin |
alpha2-7 | 5'-cccgacctctacatgactcac-3' | putative α-defensin |
alpha2-8 | 5'-cctgacctccagatgactgac-3' | putative α-defensin |
alpha2-9 | 5'-cctgaactccaggtgactsac-3' | putative α-defensin |
alpha2-10 | 5'-cctgagctccaggtgactyac-3' | putative α-defensin |
alpha2-11 | 5'-cctgagctccaggtgactccc-3' | putative α-defensin |
Oligo-dT-Bio | 5'-actctatgagaattcgatgagcgatctgt(25)v-3' | poly-A-tail |
Tail-Primer3' | 5'-actctatgagaattcgatgagcgatctg-3' | Oligo-dT-Bio |
T7 promoter-oligo | 5'-gtaatacgactcactatag-3' | pDrive cloning vector |
SP6 promoter-oligo | 5'-catttaggtgacactatag-3' | pDrive cloning vector |