Skip to main content

Table 1 Oligonucleotides for reverse-transcriptase PCR and sequencing reactions.

From: The repertoire of equine intestinal α-defensins

Name Sequence Target
alpha1 5'-gtgactsacggccatgaggac-3' putative α-defensin
alpha2 5'-gtgactsacagccatgaggac-3' putative α-defensin
alpha3 5'-gtgactsatatccatgaggac-3' putative α-defensin
alpha4 5'-gtgactsatatacatgaggac-3' putative α-defensin
alpha5 5'-atgactcacagccatgaagac-3' putative α-defensin
alpha6 5'-gtgacccacagccatgaggac-3' putative α-defensin
alpha7 5'-gtgactgacagccataaggac-3' putative α-defensin
alpha2-1 5'-cctgacctccaggtgacccac-3' putative α-defensin
alpha2-2 5'-cctgacctccagttgactccc-3' putative α-defensin
alpha2-3 5'-attcacttccaggtgactcac-3' putative α-defensin
alpha2-4 5'-ctggaactccaggtgactcac-3' putative α-defensin
alpha2-5 5'-tctgaactccaggtgactcac-3' putative α-defensin
alpha2-6 5'-ggcctcagtcaggtgactgac-3' putative α-defensin
alpha2-7 5'-cccgacctctacatgactcac-3' putative α-defensin
alpha2-8 5'-cctgacctccagatgactgac-3' putative α-defensin
alpha2-9 5'-cctgaactccaggtgactsac-3' putative α-defensin
alpha2-10 5'-cctgagctccaggtgactyac-3' putative α-defensin
alpha2-11 5'-cctgagctccaggtgactccc-3' putative α-defensin
Oligo-dT-Bio 5'-actctatgagaattcgatgagcgatctgt(25)v-3' poly-A-tail
Tail-Primer3' 5'-actctatgagaattcgatgagcgatctg-3' Oligo-dT-Bio
T7 promoter-oligo 5'-gtaatacgactcactatag-3' pDrive cloning vector
SP6 promoter-oligo 5'-catttaggtgacactatag-3' pDrive cloning vector