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Table 1 Host origin, treatment, and number of individuals used for construction of the four stage/tissue specific libraries.

From: Sympatric ecological speciation meets pyrosequencing: sampling the transcriptome of the apple maggot Rhagoletis pomonella

Stage/Tissue

Samples in each library

Host race

N

Larva

L2+L3, in fruit

Apple

16

 

L3, migrant

Apple

6

Pupa

3 days at 25°C

Hawthorn

8

 

10 days at 25°C

Hawthorn

8

 

20 days at 25°C

Hawthorn

8

 

22 days at 25°C

Hawthorn

8

 

3 days at 4°C

Hawthorn

6

 

1 months at 4°C

Hawthorn

6

 

3 months at 4°C

Hawthorn

8

 

4 months at 4°C

Hawthorn

8

 

3 days at 25°C after diapause

Hawthorn

8

 

7 days at 25°C after diapause

Hawthorn

8

 

24 days at 25°C after diapause

Hawthorn

8

 

40 days at 25°C after diapause

Hawthorn

8

Body (no head)

non-diapaused, 3 days after eclosion

Hawthorn

20

 

non-diapaused, 10 days after eclosion

Hawthorn

16

 

Wild-caught

Apple

20

Head

non-diapaused, 3 days after eclosion

Hawthorn

98

 

non-diapaused, 10 days after eclosion

Hawthorn

91

 

wild caught

Apple

51