Figure 2

Comparison of huC expression in morphants of groups II, III and IV zebrafish DUBs at 24 hpf. huC expression at 24 hpf, dorsal view. Decreased huC expression was found in morphants of these 3 groups. Group II has 16 members that showed a typical decreased huC expression (arrows). Group III has 13 members that showed the decreased huC expression with destructive patterning. Lastly, group IV has 4 members that had the most severe decreased and destructive huC expression. All 4 members named otud4, usp5, usp15 and usp25 could not form a normal neuronal pattern in the head region. Arrowheads mark the abnormal patterns in morphants of group III; while arrows indicate the absence of neurons in morphants of group IV.