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Figure 5 | BMC Genomics

Figure 5

From: Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development

Figure 5

Frequency of dorsalized and ventralized phenotypes after knockdown of group IV DUB genes and overexpression of Bmp signaling genes. Panels A to D showed the frequency of dorsalized phenotypes after knockdown of group IV DUB genes. Noted that severe dorsalized phenotypes were found after double-knockdown of usp5+usp25 or usp15+usp25 (C, D). Panels E to G showed the phenotypes' frequencies after overexpression of bmp4 and group IV DUB genes knockdown. Noted that there were phenotypic shift from ventralization to dorsalization after double knockdown of two DUB genes, with more obvious changes in otud4+usp25 (E) and usp5+usp25 (F) double knockdown. Panels H to J showed the results of the smad1 overexpression and double knockdown of group IV DUB genes. Noted that the ventralized effects were maintained in the double knockdown of group IV DUB genes. Collectively, the results suggested that group IV DUB genes are involved in the BMP pathway and function between Bmp4 and Smad1. Noted that there were three different Y axis scales in the figure (A-D; E-G; H-J). The number of injected embryos was showed in Table 1.

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