Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development

BMC Genomics

Table 1 Dorsal-ventral value of RNA and/or MO injection studies

RNA/MO	pg/em	pmol	n ep	n	DV value
-/otud4		0.5	3	104	3.14
-/usp5		2.0	3	101	2.50
-/usp15		0.5	3	127	2.10
-/usp25		2.0	3	185	3.14
-/otud4+usp25		0.25/1.0	2	100	2.59
-/usp5+usp25		1.0/1.0	2	110	3.63
-/usp15+usp25		0.25/1.0	2	78	2.56
bmp4/-	2.5		3	98	-2.66
bmp4/otud4	2.5	0.5	3	71	-2.93
bmp4/usp5	2.5	2.0	3	65	-2.52
bmp4/usp15	2.5	0.5	3	78	-2.55
bmp4/usp25	2.5	2.0	3	82	-2.54
bmp4/otud4+usp25	2.5	0.25/1.0	2	93	1.27
bmp4/usp5+usp25	2.5	1.0/1.0	2	118	2.63
bmp4/usp15+usp25	2.5	0.25/1.0	2	132	-0.31
smad1/-	800		3	96	-1.37
smad1/otud4	800	0.5	3	103	-1.44
smad1/usp5	800	2.0	3	111	-1.32
smad1/usp15	800	0.5	3	104	-0.94
smad1/usp25	800	2.0	3	98	-1.76
smad1/otud4+usp25	800	0.25/1.0	2	68	-1.08
smad1/usp5+usp25	800	1.0/1.0	2	71	-0.95
smad1/usp15+usp25	800	0.25/1.0	2	75	-0.88

Two mRNA (bmp4 and smad1) were co-injected with the group IV MOs (otud4, usp5, usp15 and usp25). The dorsal-ventral values were calculated as follows: Frequency of phenotypes * [C5: 5; C4: 4; C3: 3; C2: 2; C1: 1; Wt: 0; V1: -1; V2: -2; V3: -3; V4: -4]. The highest DV value is 5, which indicates 100% C5 phenotypes, while lowest is -4, which represents 100% V4 phenotypes. Negative values (ventralized phenotypes) were shown in bold.
Abbreviations: n ep, number of experiments; n, number of scored embryos; DV value, dorsal-ventral value.

ISSN: 1471-2164