Figure 1

Real time qRT-PCR results for genes used to validate microarray data. The standard 2^-ΔΔCT method was used to determine mean fold changes in gene expression [116]. All Ct values were normalised using the NSUN6 gene. The Student's t-test was used to identify significant differences in mRNA abundance between time-points. Each point on the graph represents the relative fold change in gene expression compared to pre-exercise levels. * signifies a P-value of <0.05 **, signifies a P-value of <0.01, *** signifies a P-value of <0.001