Genome-wide dissection of globally emergent multi-drug resistant serotype 19A Streptococcus pneumoniae

BMC Genomics

Table 6 β-lactam resistance genes pbp1a (penicillin), pbp2b (amoxicillin), and pbp2x (ceftriaxone) from reference strain R6 (Genbank AE007317), representative CBSN isolates, US isolates, and South Korean isolates.

	Changes in amino acids of conserved PBP sites
	pbp 1a			pbp 2x				pbp 2b
Strain	370-373	428-432	557-559	337-340	394-397	400-401	546-549	385-388	442-445	614-616
19F-14	SAMK		KTG	SAMK	HSSN	MS
MOH55	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
MOH56	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
MOH147	STMK	SRNVP	KTG	SAMK	HSSN	MT	LKSG	SVVK	SSNA	KTGTA
MOH83	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
MOH62	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
Kor914	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
Kor21	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
Kor39	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
PU1175	SSMK	SRNVT	KTG	SAMK	HSSN	MS	VKSG	SVVK	SSNA	KTGTG
PU6055	SSMK	SRNVT	KTG	SAFK	HSSN	TS	VKSG	SVVK	SSNA	KTGTG

Serotype (SERO), sequence type (ST), clonal complex (CC) and MIC values (mg/mL) for penicillin (PEN), ceftriaxone (CTX) and amoxicillin (AMOX) are indicated. Residues associated with resistance to β lactams are in bold letters. Ceftriaxone MIC values for South Korea isolates were unavailable. Of note, Taiwan 19F-14 (ST236) [Genbank E043521/E043522] retains residues similar to 19F and 19A in this study at certain residues of pbp1a and pbp2x. For the complete data set, including Tn2010-related resistance genes, see Additional file 3.

ISSN: 1471-2164