Figure 4
From: MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana
MicroRNA induced cleavage of At3g19890; detection of cleavage products by modified 5' RACE. A. 5' RACE to identify miR774a-directed cleavage products. B. Watson-Crick base pairing and pattern of cleavage of At3g19890 by miR774a. C. Gel image showing products of 5' RACE reaction to detect ath-MIR2939-directed cleavage. D. Watson-Crick base pairing and pattern of cleavage of At3g19890 by ath-MIR2939. E. RT-PCR detection of mature ath-MIR2939 in mature pollen (MP) and leaf (L). For A and C, Lanes C1 and C2 are controls (minus 5' RACE adapter primer and minus gene specific primer). L, DNA ladder.