Figure 2

Expression of snoRNA in P. falciparum. A) Northern hybridization of snoRNAs in P. falciparum 3D7. Total RNA was size fractionated on 10% urea-polyacrylamide gels. Blots were probed using labelled DNA primers. T and M stands for total RNA and molecular weight marker respectively. B) Reverse-transcriptase PCR assay for PFS14 using forward primer PFS14_F and reverse primer PFS14. P is the positive control containing genomic DNA as template, C1 is a negative control that lacks a template, C2 is a negative control containing cDNA generated using DNA polymerase and R is the reaction that contains cDNA generated using reverse transcriptase enzyme. L is the DNA ladder.