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Table 1 Plectus murrayi EST summary

From: Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags

Total number of high quality sequences 2,486
Average length of sequences (bp) 545 ± 156
Number of contigs§ 324
Number of singletons 1,063
Number of putative unique transcripts 1,387
Unique transcripts with similarity to C. elegans database 523 (38%)*
Unique transcripts with similarity to other nematode database 106 (7%)
Unique transcripts with similarity to other organisms 153 (11%)
Total unique transcripts with significant similarity 782 (56%)
Unique transcripts with no significant similarity 605 (44%)
  1. A sequence is considered high quality if it's trimmed PHRED 20 length is >100 bases after vector only, low-quality and contaminating sequences are removed.
  2. Calculated from the total ESTs.
  3. §A contig (contiguous sequence) contains two or more ESTs.
  4. Number of putative unique transcripts equals the number of contigs plus the number of singletons.
  5. *Calculated as percentage of total unique transcripts with similarity at E < 10-5.