Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags

BMC Genomics

Table 1 Plectus murrayi EST summary

Total number of high quality sequences^‡	2,486
Average length of sequences (bp)^†	545 ± 156
Number of contigs^§	324
Number of singletons	1,063
Number of putative unique transcripts^¶	1,387
Unique transcripts with similarity to C. elegans database	523 (38%)*
Unique transcripts with similarity to other nematode database	106 (7%)
Unique transcripts with similarity to other organisms	153 (11%)
Total unique transcripts with significant similarity	782 (56%)
Unique transcripts with no significant similarity	605 (44%)

^‡A sequence is considered high quality if it's trimmed PHRED 20 length is >100 bases after vector only, low-quality and contaminating sequences are removed.
^†Calculated from the total ESTs.
^§A contig (contiguous sequence) contains two or more ESTs.
^¶Number of putative unique transcripts equals the number of contigs plus the number of singletons.
*Calculated as percentage of total unique transcripts with similarity at E < 10^-5.

ISSN: 1471-2164