Figure 2

CREE sequence features. For illustration purposes a consensus sequence of the longer CREE is shown, here 156 bp. The ways in which the two different CREE associated promoters are generated from native sequence (blue line) and CREE sequence (red line) are shown for the Snyder promoter (left) [11] and the Black promoter (right) [10]. Both of these would drive transcription from left to right. Given favourable native sequence it might be possible to generate both promoters on the opposite strand as well, transcribing right to left. The IHF-binding site within the CREE is shown (orange), as are the inverted repeats that potentially form mRNA hairpin substrates for RNase III (green).