Figure 4
From: Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?
Result of the HTS conjugation assay when applied to a selected subset of the 20,000 mini-Tn 10 ::Km insertion mutants. The HTS conjugation assay was run on a collection of 20,000 mini-Tn10::Km insertion mutants as described in Methods. A subset of 237 mutants, which gave ALU ≤ -1 in the first assay were selected for a second assay. The figure shows the ALU values [ALU = log10 (Light-x/Light-wt)] of these 237 mutants plotted in increased order (grey diamonds), together with donor without recipient (UCDPM1; black dash) and DH5α expressing Eex_R388 [DH5α (pSU5024); black circle].