Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?

BMC Genomics

Table 1 Conjugation frequencies of a selected subset of the Keio collection mutants

Recipient strain	Gene mutated and relevant features	ALU^a	Relative transfer frequency in standard assay (% of wt)^b
No recipient		-4.37	<10^-6
BW25113 (pSU5024)		-2.55	<10^-6
K1:F4	ihfB: Integration host factor, beta subunit	-1.44	98
K83:B10	rfaC: Inner core LPS biosynthesis	-1.28	22
K29:D12	uvrD: Subunit × of helicase II (DNA repair enzyme)	-1.25	41
K69:C4	rimM: 16S rRNA processing protein	-1.03	48
K25:F8	ybeX: Putative integral membrane protein; magnesium and cobalt transporter	-1.02	55

^a Arbitrary light units produced by the given mutant in the HTS conjugation assay (see text for details).
^b Results of a standard 1 h plate conjugation assay (see Methods). Results are shown relative to the parental BW25113 strain. Transfer rates are the average of at least three independent experiments.

ISSN: 1471-2164