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Table 4 Results of EST assembly for each Mediterranean mussel cDNA library.

From: MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences

Library

Total ESTs

Discared ESTs

Analyzed ESTs

# EST in cluster

# clusters

# singletons

# consensus

% poly(A) detection

% gene discovery

% redundancy

DiG01

93

24

69

54

31

15

46

66.7

21.7

78.3

DiG02

95

23

72

57

28

15

43

73.6

23.6

76.4

Ese00

285

121

164

143

90

21

111

34.8

12.8

87.2

GDG01

35

10

25

19

19

6

25

64.0

24.0

76.0

Gll01

95

18

77

45

37

32

69

51.9

41.6

58.4

Hae01

656

93

563

511

237

52

289

56.0

10.3

89.7

Hae02

540

100

440

352

196

88

284

41.4

21.4

78.6

Hae03

523

130

393

354

126

39

165

83.2

10.9

89.1

Hae04

568

116

452

411

130

41

171

81.2

9.5

90.5

Hae05

475

134

341

309

110

32

142

83.3

10.0

90.0

MxT01

767

200

567

381

260

186

446

34.0

41.1

58.9

MxT02

768

577

191

138

90

53

143

39.8

37.2

62.8

MxT03

4224

1478

2746

2038

586

708

1294

50.6

38.2

61.8

MxT04

74

22

52

52

50

0

50

61.5

0.0

100.0

Nor01

15621

3034

12587

9228

2137

3359

5496

23.3

65.6

34.4

Tst00

96

55

41

25

24

16

40

53.7

39.0

61.0

Tst01

24

16

8

5

5

3

8

25.0

37.5

62.5

Overall

24939

6151

18788

14122

4156

4666

8822

54.3

26.1

73.9

  1. Total ESTs = number of produced chromatograms; Discarded ESTs = number of low quality ESTs; Analyzed ESTs = number of sequences processed for clustering; # EST in cluster = number of sequences in cluster; # clusters = total number of clusters; # singletons = number of putative transcripts identified by one single EST; # consensus = number of non-redundant sequences; % poly(A) detection = percentage of sequences where poly(A) has been identified and trimmed; % gene discovery = percentage of ESTs identifying putative new transcripts in the total EST analyzed from each library; % redundancy = percentage of sequences also identified by other cDNA libraries.