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Table 3 Primer sets used for construction of double homologous crossover cassettes

From: Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production

   Forward primer Reverse primer
Flank Size (kb) ID primer Introduced restriction site ID primer Introduced restriction site
Left 7 5 Acc65I 6 Not I
Left 5 7 Acc 65I 6 Not I
Left 3 8 Acc 65I 6 Not I
Right 3 9 NotI 10 Eco52I, Acc65I
Right 5 9 Not I 11 Eco 52I, Acc 65I
Right 7 9 Not I 12 Eco 52I, Acc 65I