|  | Forward primer | Reverse primer | ||
---|---|---|---|---|---|
Flank | Size (kb) | ID primer | Introduced restriction site | ID primer | Introduced restriction site |
Left | 7 | 5 | Acc65I | 6 | Not I |
Left | 5 | 7 | Acc 65I | 6 | Not I |
Left | 3 | 8 | Acc 65I | 6 | Not I |
Right | 3 | 9 | NotI | 10 | Eco52I, Acc65I |
Right | 5 | 9 | Not I | 11 | Eco 52I, Acc 65I |
Right | 7 | 9 | Not I | 12 | Eco 52I, Acc 65I |