Asymmetrical distribution of non-conserved regulatory sequences at PHOX2B is reflected at the ENCODE loci and illuminates a possible genome-wide trend

Table 2 Human PHOX2B elements conserved to zebrafish phox2b locus demonstrate activity consistent with orthologous zebrafish sequences.

Human Amplicon	Expression (HCS)	Zebrafish Amplicon	Expression (ZCS)	Coincident Control
PHOX2B-HCS+0.6*	Fb, CG	phox2b-ZCS +0.5	Hb, SC	No
PHOX2B-HCS -0.0	VDi, Hb	phox2b-ZCS +0.0	Hb, SC	Yes
PHOX2B-HCS -2.9*	Fb, Hb	phox2b-ZCS -1.4	Mb, Hb, SC	Yes
PHOX2B-HCS -73.5	OT, CG, Hb	phox2b-ZCS -8.3	Mb, Hb, CG	Yes
PHOX2B-HCS -108.3	Fb, Hb	phox2b-ZCS -16.6	Mb, Hb	Yes
PHOX2B-HCS-114.8	Fb, Hb, SC	phox2b-ZCS-20.1	Hb	Yes
PHOX2B-HCS -116.7	CG	phox2b-ZCS -23.7	Hb, SC, ENS	No
PHOX2B-HCS -130.4	ENS	phox2b-ZCS -23.7	Hb, SC, ENS	Yes

HCS expression is pattern driven by human amplicon in G0 zebrafish embryos (* indicates G1 expression pattern). ZCS expression is pattern driven by orthologous zebrafish amplicon in G1 zebrafish embryos [29]. Overlap is categorized as Yes, tissue overlap in expression patterns, but additional tissues seen; No, no overlap in expression. Fb, Forebrain; OT, Oculomotor and trochlear motor Progenitors; VDi, Ventral diencephalon; Hb, Hindbrain; CG, Cranial ganglia,; SC, Spinal cord ENS, Enteric nervous system.

ISSN: 1471-2164