Table 5 Ingenuity biological function analyses of IL-6-versus NGF-regulated genes in PC12 cells (selected)

Cellular Growth and Proliferation

Growth of cells

2.27 × 10-4

ACVR2A, AHSG, ANXA1, BCL2L11, BRCA1, CASP1, CDC42, CHRM3, CXCL10, EGR1, FGFR1, GAP43, GDF15, GHR, GRID2, HGF, IGF2R, IRF1, ITGA7, JAK2, MAP2K5, MST1, MT3, MX1, NOS3, NOS2A, PIM3, RARA, SCAMP2, SDC2, STAT1, TIMP1, VDR

8.82 × 10-3

ACHE, AGTR1, BDNF, BNIP3, CASP1, CD44, CHRM3, CREM, DDR1, DUSP6, FBN2, FN1, GDF15, GJB2, GRID2, MYL9, NRG1, PDIA3, PTPN11, SLC30A1, TGFB1, TPM1, VPS33B, ZMAT3

Proliferation of cells

9.06 × 10-7

ACVR2A, ADCYAP1, ANXA1, AVP, BCL2L11, BRCA1, CALCB, CDC42, CHRM3, CHRM4, CRYAB, CXCL10, EGR1, FGFR1, FRK, GDF15, GHR, GHRH, HGF, IGF2R, IRF1, JAG2, JAK2, KLF6, KLK8, LCN2, MAP2K5, MT3, NFIB, NOS3, NOS2A, NR3C2, PDGFA, RARA, REG1A, REG3A, RNMT, SDC2, ST6GAL1, STAT1, TIMP1, TPM3, USF1, VDR, VIPR1

3.82 × 10-3

AGTR1, AKR1C3, BDNF, CALCA, CD44, CHRM3, DDR1, FN1, GDF15, GRK5, NPPC, NRG1, PPIA, PTPN11, TAC1, TGFB1

Cellular Movement

Cell movement

2.18 × 10-8

ADCYAP1, ANXA1, CASP1, CDC42, CHRM3, CHRM4, CXCL10, CXCL13, EGR1, FCGR2A, FER, FGB, FGFR1, GNAZ, GRID2, HGF, HLA-G, HSP90AB1, IGF2R, JAK2, LCN2, LGALS9, LIMS1, MAP2K5, MST1, NOS3, NOS2A, OLR1, PDGFA, RARA, REG3A, SDC2, ST6GAL1, STAT1, TIMP1, TPM3, TUBB, VDR, VIPR1

7.96x10-5

ADAM17, AGTR1, APCS, BDNF, CALCA, CASP1, CD44, CHRM3, DDR1, FN1, GJB2, GNAZ, GRID2, LCAT, LIMS1, NAP1L4, NPPC, NRG1, PDE4B, PPIA, PTPN11, SCN2B, SELP, SLC1A3, TAC1, TGFB1, TPM1

Chemotaxis

4.05 × 10-4

ANXA1, CDC42, CXCL10, CXCL13, FCGR2A, FER, FGFR1, GNAZ, HGF, IGF2R, LGALS9, NOS3, VIPR1

6.29x10-5

AGTR1, BDNF, CALCA, CD44, FN1, GNAZ, NAP1L4, PDE4B, PPIA, PTPN11, SCN2B, TAC1, TGFB1

Cell-To-Cell Signaling and Interaction

Adhesion of cells

1.47 × 10-7

ANXA1, CDC42, CDH17, CXCL10, EGR1, FCGR2A, FER, FEZ1, FGB, FGFR1, FGG, GRID2, HGF, IGF2R, ITGA7, JAG2, LGALS9, LIMS1, NOS3, OLR1, REG3A, SDC2, ST6GAL1, STAT1, STX4, TIMP1

1.34x10-4

ACHE, ADAM17, CASK, CD44, CNTN4, DDR1, DLG1, FGG, FN1, GRID2, LIMS1, NRG1, PTPN11, SELP, STX4, TAC1, TGFB1, TPH1

Cell Signaling

Quantity of calcium

3.25 × 10-3

ADCYAP1, AVP, CHRM3, CXCL10, CXCL13, FCGR2A, GHRH, HGF, NOS3, NOS2A, VDR

8.89x10-6

AGTR1, BDNF, CALCA, CHRM3, FN1, GRK5, NPPC, PLD2, PPIA, PTHR1, PTPN11, SELP, TAC1, TGFB1

Production of nitric oxide

1.33 × 10-3

IRF1, JAK2, MST1, NOS3, NOS2A, STAT1

-

-

Flux of calcium

1.67 × 10-3

ADCYAP1, ANXA1, AVP, CHRM3, CXCL10, CXCL13, FCGR2A, P2RX2

2.20x10-3

CALCA, CHRM3, FN1, NPPC, P2RX2, PPIA, TGFB1

Cell surface receptor linked signal transduction

1.45 × 10-3

ACVR2A, ANXA1, CDC42, CXCL10, FCGR2A, FGFR1, ITGA7, JAK2, KLF6, LIMS1, PDGFA, PTPRD, STAT1

-

-

Small Molecule Biochemistry

Quantity of cyclic AMP

1.00 × 10-5

ADCYAP1, AVP, CHRM4, CXCL10, GAP43, GHRH, GNAZ, NOS3, VIPR1

6.03x10-3

BDNF, CALCA, GNAZ, NPPC, PTHR1

Production of cyclic AMP

2.17 × 10-4

ADCYAP1, AVP, GHRH, GNAZ, NOS3, NOS2A, VIPR1

Accumulation of cyclic AMP

1.21 × 10-3

ADCYAP1, AVP, AVPR2, CHRM3, GHRH, VIPR1

4.35 × 10-4

CALCA, CHRM3, GRK5, PTHR1, TAC1, TGFB1

Formation of cyclic AMP

1.28 × 10-4

ADCYAP1, AVP, AVPR2, GHRH, GANZ

7.26 × 10-4

CALCA, GNAZ, PTHR1, TAC1

Release of Ca2+

9.82 × 10-5

ANXA1, AVP, CHRM3, FCGR2A, FGB, FGG

-

-

Quantity of cholesterol

-

-

2.85 × 10-3

ATP7B, BDNF, CALCA, GULO, LCAT

Post-Translational Modification

Modification of protein

1.57 × 10-5

AVP, BRCA1, CASP1, CHRM3, FCGR2A, FER, FGFR1, GRID2, HSP90AB1, HTATIP, JAK2, LHB, MST1, NOS3, NOS2A, PDGFA, PDIA2, PDP2, PIM3, PTPRD, ST6GAL1, STAT1, TGM1

4.47 × 10-3

APCS, CASP1, CD44, CHRM3, DUSP6, FN1, GRID2, NDST1, NRG1, PDIA3, PPIA, PTPN11, RABGGTA, TAC1, UBB

Nervous system development and function

growth of neurites

8.02 × 10-3

ADCYAP1, CDC42, GAP43, HGF, TPM3

-

-

survival of neurons

3.60 × 10-3

ADCYAP1, BCL2L11, GDF15, HGF, RARA, REG3A

-

-

development of synapse

6.57 × 10-3

GRID2, NFASC

-

-

fasciculation of axons

3.14 × 10-2

GAP43

-

-

complexity of dendritic trees

1.25 × 10-2

HGF

-

-

long-term potentiation of dentate gyrus

1.25 × 10-2

EGR1

-

-

neurological process of synapse

-

-

1.60 × 10-4

BDNF, CHRM3, CHRNB1, NRG1, PPP1R1A

synaptic transmission

-

-

2.88 × 10-4

BDNF, CACNB2, CHRM3, CHRNB1, GABRG2, P2RX2, SCN2B, SLC1A1, SLC1A3

neurological process of axons, neurites

-

-

4.79 × 10-4

BDNF, CNTN4, GRID2, NRG1, PDIA3, UBB

activation of nerves

-

-

7.73 × 10-4

CALCA, TAC1

binding of neurites

-

-

7.73 × 10-4

BDNF, CD44

size of cell body

-

-

7.73 × 10-4

ACHE, BDNF

survival of neurons

-

-

8.92 × 10-4

BDNF, GDF15, NRG1, PDIA3, SLC1A3, TGFB1

development of neurites

-

-

2.83 × 10-3

ACHE, BDNF, GRID2, NRG1, PDIA3, PTPN11

migration of nervous tissue cell lines

-

-

3.38 × 10-3

NRG1, TGFB1

proliferation of nervous tissue cell lines

-

-

6.67 × 10-3

NPPC, TGFB1