Table 3 RNAi system with transcription/translation machinery

1

mRNA + siRNA -> gRNA

0.008 hr^-1

2

dsRNA -> 10*siRNA

2.0 hr^-1

3

mRNA -> dsRNA

0.002 hr^-1

4

siRNA ->ϕ

2.0 hr^-1

5

mRNA ->ϕ

18 hr^-1

6

gRNA ->ϕ

2.8 hr^-1

7

O + R -> O_R

12.0 hr^-1

Regulatory molecule R binds to the operator region O to form the bound complex O_R

8

O_R -> O + R

0.24 hr^-1

O_R dissociates into free R and O

9

P + RNAP -> P_RNAPC

1.2 hr^-1

RNAP binds to promoter region P forming closed complex P_RNAPC

10

P_RNAPC -> P + RNAP

0.06 hr^-1

P_RNAPC dissociates into free RNAP and P

11

P_RNAPC -> P_RNAPO

48 hr^-1

Isomerization of closed to open complex (P_RNAPO)

12

P_RNAPO -> TrRNAP + mRNA + P

54 hr^-1

RNAP clears promoter region and mRNA chain synthesis starts. TrRNAP denotes transcribing RNA polymerase.

13

TrRNAP -> RNAP

4.8 hr^-1

RNAP completes transcription and is released from DNA.

14

mRNA + Ribosome -> RibRBS

0.6 hr^-1

Ribosome binds to mRNA forming bound complex RibRBS

15

RibRBS -> mRNA+ Ribosome

0.06 hr^-1

Ribosome dissociation from RibRBS

16

RibRBS -> EIRib + mRNA

18 hr^-1

Ribosome EIRib initiates translation of mRNA chain

17

EIRib -> protein

48 hr^-1

Protein synthesis by transcribing ribosome

18

protein ->ϕ

0.18 hr^-1

Protein product degradation