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Table 3 RNAi system with transcription/translation machinery

From: Comparing 2-nt 3' overhangs against blunt-ended siRNAs: a systems biology based study

Reaction Number Reaction Rate Constant Remarks
1 mRNA + siRNA -> gRNA 0.008 hr-1  
2 dsRNA -> 10*siRNA 2.0 hr-1  
3 mRNA -> dsRNA 0.002 hr-1  
4 siRNA ->ϕ 2.0 hr-1  
5 mRNA ->ϕ 18 hr-1  
6 gRNA ->ϕ 2.8 hr-1  
7 O + R -> O_R 12.0 hr-1 Regulatory molecule R binds to the operator region O to form the bound complex O_R
8 O_R -> O + R 0.24 hr-1 O_R dissociates into free R and O
9 P + RNAP -> P_RNAPC 1.2 hr-1 RNAP binds to promoter region P forming closed complex P_RNAPC
10 P_RNAPC -> P + RNAP 0.06 hr-1 P_RNAPC dissociates into free RNAP and P
11 P_RNAPC -> P_RNAPO 48 hr-1 Isomerization of closed to open complex (P_RNAPO)
12 P_RNAPO -> TrRNAP + mRNA + P 54 hr-1 RNAP clears promoter region and mRNA chain synthesis starts. TrRNAP denotes transcribing RNA polymerase.
13 TrRNAP -> RNAP 4.8 hr-1 RNAP completes transcription and is released from DNA.
14 mRNA + Ribosome -> RibRBS 0.6 hr-1 Ribosome binds to mRNA forming bound complex RibRBS
15 RibRBS -> mRNA+ Ribosome 0.06 hr-1 Ribosome dissociation from RibRBS
16 RibRBS -> EIRib + mRNA 18 hr-1 Ribosome EIRib initiates translation of mRNA chain
17 EIRib -> protein 48 hr-1 Protein synthesis by transcribing ribosome
18 protein ->ϕ 0.18 hr-1 Protein product degradation