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Table 1 Higher ΔGas-5 increases the probability that shRNAs are functional.

From: Energy profile and secondary structure impact shRNA efficacy

shRNA ΔGas-5 Number Functional Rate
>= -3.2 Functional 155 81.6% (155/190)
  Not functional 35  
<-3.2 Functional 66 66.7% (66/99)
  Not functional 33