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Table 3 Sequence characteristics that consistently show either positive or negative impacts on shRNA efficacy in Least Square Analysis.

From: Energy profile and secondary structure impact shRNA efficacy

Positive Parameters
Parameter Position Coefficient
G 3 8.01
G 5 9.11
A 11 7.59
T 14 6.16
CA 6–7 11.48
CC 8–9 11.63
TG 10–11 19.58
GA 13–14 31.32
TA 17–18 6.36
TA 18–19 24.11
Negative Parameters
Parameter Position Coefficient
TA 4–5 -35.04
TA 6–7 -23.35
GG 8–9 -21.00
GC 9–10 -24.66
GA 17–18 -15.08