Table 3 Sequence characteristics that consistently show either positive or negative impacts on shRNA efficacy in Least Square Analysis.
From: Energy profile and secondary structure impact shRNA efficacy
Positive Parameters | ||
|---|---|---|
Parameter | Position | Coefficient |
G | 3 | 8.01 |
G | 5 | 9.11 |
A | 11 | 7.59 |
T | 14 | 6.16 |
CA | 6–7 | 11.48 |
CC | 8–9 | 11.63 |
TG | 10–11 | 19.58 |
GA | 13–14 | 31.32 |
TA | 17–18 | 6.36 |
TA | 18–19 | 24.11 |
Negative Parameters | ||
Parameter | Position | Coefficient |
TA | 4–5 | -35.04 |
TA | 6–7 | -23.35 |
GG | 8–9 | -21.00 |
GC | 9–10 | -24.66 |
GA | 17–18 | -15.08 |