Semiquantitative analysis of CYP2D6 copy number variations. Successful penta-plex PCR products of CYP2D6 analysis were shown. Lanes 1-5 show amplification of the CYP2D6 exon sequences, LDL, and DMD from different human samples using five primer pairs listed in Table 1. LDL is an internal standard for common two copies (autosomal gene) and DMD is internal standard for one copy in men (X-linked). 100 bp M is DNA marker. DMD = dystrophin. LDL = low density lipoprotein.