Figure 5From: Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of HumanPPARγ binds to PPRE1 in human NHE1 promoter. (A) Schematic representations of full length human NHE1 promoter construct (-1374/+16) and a 5'-deletion construct (-850/+16) with location of PPRE1 and PPRE2 indicated. (B) MCF-7 cells transiently transfected with full length human NHE1 promoter construct (-1374/+16) and a 5'-deletion derivative of the full length lacking the PPRE site (-850/+16). Cells were treated with 3 μM and 5 μM 15 d-PGJ2 for 16 h. CAT-ELISA activity measured following manufacturer's instructions. NHE1 promoter activities were calculated as CAT activity (A405 nm)/μg total protein and expressed as absorbance/μg total protein. Data represents the average +/- SD of two experiments done in duplicate.Back to article page