Figure 3

Two examples of miRNA target sites in orthologous gene transcripts. Potential target sites of miR-1 (blue arrows) in the 3'-UTR sequences of the orthologous CNN3 (A) and LARP4 (B) transcripts are shown. Predicted duplexes formed by the 3'-UTR sequences (top) and miRNAs (bottom) are shown in dotted boxes for each potential target site. The green bar on the H. sapiens 3'-UTR sequence indicates a DNA region used for the construction of the reporter plasmid pLuc-CNN3 (Figure 4A). See Additional file 3 for other candidates. (*) The length of the CNN3 3'-UTR is currently registered as a little shorter than that indicated (527 nt in size) and contains the miR-1 binding site (Ensembl release 53).