Global transcriptional analysis of nitrogen fixation and ammonium repression in root-associated Pseudomonas stutzeri A1501

Table 2 Identification of nine selected nitrogen fixation-inducible genes and nitrogenase activity of the corresponding mutant strains

Gene ID	Functional description	Involvement in nitrogen fixation in other systems	Nitrogenase activity^a, %
PST0446	Cytoplasmic membrane protein	Unknown	64
PST0874	Chemotactic transducer	Unknown	52
PST1325	conserved hypothetical protein	Unknown	23
PST1521	outer membrane protein	Unknown	68
PST2508	methyl-accepting chemotaxis transducer	Unknown	92
PST3417	predicted transcriptional regulators	Unknown	58
PST3422	outer membrane protein	Unknown	96
PST3621	transcriptional regulator, AraC family	Unknown	80
PST4084	ABC-type amino acid transport/signal transduction systems	Unknown	54

^aNitrogenase activity of nine non-polar mutants (enabling transcription of downstream genes) is expressed as % of wild type A1501 activity, which is in the range of 1645 ± 169 nmol ethylene/min/mg protein. Nitrogenase activity was examined under nitrogen fixation conditions (0.1 mM ammonium and 0.5% oxygen tension). Data are expressed as the means of three independent experiments.

ISSN: 1471-2164