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Table 1 Bacterial isolates used in this study and results of PCR-based screening assays.

From: High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak

    

Pulsed-field gel electrophoresis

Prophage Ï•LMC3b

pLM5578

   

Isolate No.

Source

Specimen typea

Serotype

Asc I

Apa I

terminase

tail protein

virD4

fic

buk c

gltX d

SNPse

08-5578

Human

Blood

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-5923

Human

Blood

1/2a

LMACI.0001

LMAAI.0001

-

-

-

-

WT

WT

27

08-6040

Food

RTE meat

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-6055

Food

RTE meat

1/2a

LMACI.0040

LMAAI.0001

+

+

-

-

FS

- 21 bp

1

08-6135

Human

CSF

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-6567

Environment

Food processing

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-6061

Food

RTE meat

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-6421

Human

Blood

1/2a

LMACI.0040

LMAAI.0001

+

+

+

+

FS

- 21 bp

1

08-5828

Human

Blood

1/2a

LMACI.0040

LMAAI.0001

+

+

-

-

FS

- 21 bp

1

08-7374

Environment

Food processing

1/2a

LMACI.0001

LMAAI.0001

-

-

-

-

FS

WT

0

08-7376

Environment

Food processing

1/2a

LMACI.0001

LMAAI.0001

-

-

-

-

FS

WT

0

08-7381

Environment

Food processing

1/2a

LMACI.0001

LMAAI.0001

-

-

-

-

FS

WT

0

08-7382

Environment

Food processing

1/2a

LMACI.0001

LMAAI.0001

-

-

-

-

FS

WT

0

  1. a. 'RTE', ready to eat; 'CSF', cerebrospinal fluid
  2. b. '+', amplicon of expected size detected using PCR using oligonucleotides described in Additional file 6; '-', no amplicon detected
  3. c. 'FS', frameshift resulting in a truncated coding sequence encoding butyrate kinase; 'WT', wild type. Confirmed by Sanger-based DNA sequencing of amplicons generated by high fidelity PCR.
  4. d. 'WT', wild type; '-21 bp', in-frame deletion of 7 codons. Confirmed by Sanger-based DNA sequencing of amplicons generated by PCR.
  5. e. Number of SNP's relative to the hypothetical last common ancestor presented in Fig. 6. Sanger-based DNA sequence confirmation of SNPs was completed after PCR amplification using oligonucleotides described in Additional file 6.