Figure 1From: Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant speciesBAC-FISH of selected BAC clones with the T. aestivum mitotic metaphase chromosomes. (A) Clone 3AS0096P02 (labeled pale orange) showed a dispersed hybridization across all 21 pairs of chromosomes and could not be localized to a genome. (B) Clone 3AS0034G08 (labeled green) hybridized with 7 pairs of chromosomes belonging to the D genome identified using a probe for the Afa repeat family (labeled red). (C and D) Clones 3DS0002N13 (C) and 3DS0019H15 (D) (both labeled green) showed dispersed signal on 7 pairs of chromosomes belonging to the A genome, which were identified by the failure to hybridize with GAA microsatellites (labeled yellow) and an Afa family repeat (labeled red). All chromosomes were counterstained with DAPI (blue).Back to article page