Figure 6

Genomic Southern blotting analysis and chromosomal distribution of TremA and TremB elements. A) Genomic DNA of isolates Pb01, Pb03 and Pb18 was digested with Bgl II, blotted onto nylon membranes and hybridized with TremA, TremB and TremE probes derived from the internal region of each element. B) Chromosomal distribution of TremA and B elements in different P. brasiliensis isolates. On the left are the molecular karyotypes of P. brasiliensis isolates Pb01, Pb03 and Pb18. Chromosomal bands were separated by pulsed-field gel electrophoresis and stained with EtBr. The autoradiograms from Southern hybridization using the elements TremA and TremB as probes are shown on the right.