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Figure 1 | BMC Genomics

Figure 1

From: Whole genome analysis of p38 SAPK-mediated gene expression upon stress

Figure 1

p38 SAPK activation in wt MEFs and p38α-/- knock out MEFs. A. wt and p38α-/- MEFs cells were stimulated with 100 ng/ml TNFα, 25 ng/ml anisomycin and 100 mM NaCl for the indicated times. Cleared cell lysates were subsequently analysed by western blotting with antibodies that recognise the phosphorylated and active form of p38 SAPK, total p38α SAPK or tubulin. Representative western blots are shown. B. wt MEFs were pre-treated or not with 10 μM SB203502 prior to the addition of the p38 SAPK activators for the indicated times. Cleared cell lysates were subsequently analysed by western blotting with antibodies that recognise phosphorylated MK2, the phosphorylated and active form of p38 SAPK and total p38α SAPK. Representative western blots are shown. C. As in B western blots were analysed with antibodies raised against total and phosphorylated MK2, pp38 SAPK and tubulin. Rrepresentatives western blots are shown.

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