Analysis of Yap1-GFP localization in CTBT-treated cells. Exponentially growing yeast cells (EG103) were exposed for indicated time to CTBT (4 μg/ml) and hydrogen peroxide (4 mM) as a positive control. Nuclear localization of Yap1-GFP was verified by co-localization with nuclei stained with DAPI (2 μg/ml) (A). For each sample, at least 100 cells were scored for subcellular localization of Yap1-GFP. Open bars represent cells with cytoplasmic Yap1-GFP, hatched bars represent cells with nuclear Yap1-GFP (B). Data are presented as the average of the two independent experiments.