Figure 5

Superoxide production and growth inhibition in CTBT treated cells. Fluorescence of oxidized MitoSOX Red was determined using fluorescence spectrometry (A) and fluorescence microscopy (B). The values represent the means of 3 independent experiments. Open bars represent cells grown without CTBT. Wild-type cells (BY4741) were grown in the presence of 2 μg/ml (hatched bars) and 4 μg/ml (filled bars) CTBT. sod1Δ mutant cells were grown in the presence of 0.25 μg/ml (hatched bars) and 0.4 μg/ml (filled bars) CTBT. sod2Δ mutant cells were grown in the presence of 0.25 μg/ml CTBT (hatched bars). (C) Growth inhibition zones of CTBT in mutant strains lacking the indicated superoxide dismutase genes was scored after 5 days incubation.