Table 3 Primers used for probes, screening and real time PCR
From: Comprehensive analysis of MHC class I genes from the U-, S-, and Z-lineages in Atlantic salmon
Sequence (5'-3') | Usage | |
|---|---|---|
SAA_387F1 | TATGAGCCATGCATATGAC | cDNA amplification of SAA |
SAA_815R1 | CTGTAGCTCTGGGTGTCCT | cDNA amplification of SAA |
UHA_329F1 | TGCAGAAAATGTACAGCTG | cDNA amplification of UHA |
UHA_798R1 | GTAGTTGTGCTGCTGTAGG | cDNA amplification of UHA |
ZE_481F1 | TTGAAATTCATAAAGGGCAC | cDNA amplification of ZE |
ZE_909R1 | GGGATCTGCACACTCATC | cDNA amplification of ZE |
114I13-F2 | TGATTCCCATCTCAGTATCC | Marker Ssa10083BSFU in BAC 114L13 |
114I13-R | TAGCTACCTTTCTGAGCCTG | Marker Ssa10083BSFU in BAC 114L13 |
184H23-F2 | CAGGCCTTTACTCTCTGCTA | Marker Ssa10084BSFU in BAC 184H23 |
184H23-R | GGGAGTGTTGTCTAAACCTG | Marker Ssa10084BSFU in BAC 184H23 |
21M13 | TGTAAAACGACGGCCAGT | |
UBA-F | CTGACAACTCTGGGAGAGCT | Realtime PCR Sasa-UBA |
UBA-R | GTGTGTTATGTTCTTGAGACGT | Realtime PCR Sasa-UBA |
439_UDA_967F | CCATCATTGTCCCCATCATTG | Realtime PCR Sasa-UDA |
439_UDA 1094R | AGATCTTTCCCAGAGTACACA | Realtime PCR Sasa-UDA |
UGA-F | GTGAATGATCAGGTCATTAGCC | Realtime PCR Sasa-UGA |
UGA-R | CTCTGCGCCTCTCAGGTCC | Realtime PCR Sasa-UGA |
UHA1_800F | TACAGCAGCACAACTACACAT | Realtime PCR Sasa-UHA1 |
UHA1_920R | TGACCAATACCAAGACCATGTAGA | Realtime PCR Sasa-UHA1 |
UHA2end-F | TACACATGCACTGTCCAACAC | Realtime PCR Sasa-UHA2 |
UHA2end-R | GCCAATGTGACCAATACAATGG | Realtime PCR Sasa-UHA2 |
ULA_961F | TCAAGACCAACTGGGGAAACA | Realtime PCR Sasa-ULA |
ULA_1094R | CCGTCTTTCTTCTCTTGTCTG | Realtime PCR Sasa-ULA |
ZAA_847F | CCAGAGGCAGACAAGGAAAC | Realtime PCR Sasa-ZAA |
ZAA 954R | CAGCCCCAATGACTACAGCA | Realtime PCR Sasa-ZAA |
ZBA_840F | CCCAGAGGCAGACAAGGACA | Realtime PCR Sasa-ZBA |
ZBA_949R | AATTAGAGAGGCCTGGATCCC | Realtime PCR Sasa-ZBA |
ZCA_842F | CAGAGGCAGACAAGCAAACC | Realtime PCR Sasa-ZCA |
ZCA_963Rseq | ATTGATAACCATGCAAATAACTGG | Realtime PCR Sasa-ZCA |
SAA_379F | CCTTTATGAGCCATGCATATGA | Realtime PCR Sasa-SAA |
SAA_504R | AATGAATGACCAGCCTAACAAG | Realtime PCR Sasa-SAA |