Unified translation repression mechanism for microRNAs and upstream AUGs

BMC Genomics

Table 3 Genes containing uAUGs predicted to interact with expressed miRNAs

Gene	uAUG^†	Cell line used in experiments	miRNAs predicted to interact^§	Evidence of miRNA expression^‡
MOR	gcccAUGcucc (1×)	HEK293	hsa-miR-146a (3')	No
			hsa-miR-202 (3')	No
	ggggAUGcuaa (2×)		hsa-miR-324-5p (5')	Yes[42]
			hsa-miR-517b (5')	Yes[42]
	aaggAUGcgcc (3×)		hsa-miR-323-5p (3')	No
			hsa-miR-324-5p (5')	Yes[42]
			hsa-miR-450b-3p (3')	No
CHOP	uaucAUGuuaa (1×)	HeLa	None
	aaagAUGagcg (6×)		hsa-miR-574-3p (5')	Yes[41, 42]
			hsa-miR-556-3p (3')	No
	gcagAUGugcu (2×)		hsa-miR-219-2-3p (3')	No*
MDM2	aaagAUGgagc (3×)	HeLa	hsa-miR-363 (3')	Yes[42]
	uggaAUGaucc (1×)		None
ADH5/FDH	gcccAUGccuc (4×)	HeLa	hsa-miR-146a (3')	No
			hsa-miR-202 (3')	No
	ccggAUGucag (4×)		hsa-miR-219-1-3p (3')	No*
			hsa-miR-219-2-3p (3')	No*
			hsa-miR-487a (3')	No
			hsa-miR-489 (5')	No
KLF13	cacaAUGcgcg (1×) ^#	Jurkat	hsa-miR-323-5p (3')	No
			hsa-miR-103 (5')	Yes[41]
			hsa-miR-107 (5')	Yes[41]
			hsa-miR-33a (5')	Yes[44, 45]
	ccccAUGcgcu (2×)		hsa-miR-586 (5')	No
			hsa-miR-202 (3')	No
	gcggAUGcgcg (2×)		hsa-miR-450b-3p (3')	No
			hsa-miR-324-5p (5')	Yes[41, 44]

^† uAUGs shown in caps. Strength of translation repression (1×-6×) was deduced by comparing the effect of the uAUG either to a control construct or to a construct where the uAUG under consideration is mutated or deleted.
^# uAUG not present in the GenBank entry but used in reporter constructs [26].
^§ Numbers in parentheses indicate the miRNA end predicted to interact. miRNAs in italics indicate matches with one GU wobble.
^‡ Reference for evidence of expression.
* Expression of mature miR-219, which corresponds to the 5p arm of the precursor, was detected by Chen et al., but that of 3p was not assayed for on the microarray [42].

ISSN: 1471-2164