Figure 5
Confirmation of the differential expression of selected genes in a fadD mutant of S. meliloti in response to different growth conditions as determined by quantitative real-time PCR. A) The relative expression of surface responsive genes was calculated as the fold change between growth on solid MM (black bars) or semisolid MM (white bars) compared to growth in liquid MM after 14 hours of incubation. B) The relative expression of genes responding specifically to swarming inducing conditions was calculated as the fold change between growth on semisolid MM compared to growth on hard agar MM at 7 hours (white bars) or at 14 hours (black bars). For comparisons, fold changes in gene expression obtained in the microarray experiments (calculated as 2M) are shown in parenthesis. Results are averages from at least two independent biological experiments with three technical replicates. Error bars indicate standard error at 95% confidence. nd, not detected in the microarray experiments