Figure 5

BSA expression profiling of methionine content in tubers. QTL analysis of tuber methionine content for two consecutive years (year1, 2) in the C × E population showing significant QTLs on linkage groups 3 and 5 (a). Graphical genotyping of the markers scores in the genomic region representing the methionine QTL on linkage group 3 and 5. Four bulks were constructed (3+5+, 3+5-, 3-5+, 3-5-) that represent either the presence or absence of the identified QTLs (b). Average methionine content of the four bulks was calculated based the genotypes scores (c). Hybridization scheme implementing a loop design for the four constructed bulks (d).