Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

O'Toole, Paul W; Snelling, William J; Canchaya, Carlos; Forde, Brian M; Hardie, Kim R; Josenhans, Christine; Graham, Robert LJ; McMullan, Geoff; Parkhill, Julian; Belda, Eugenio; Bentley, Stephen D

doi:10.1186/1471-2164-11-164

BMC Genomics

Table 4 The envelope proteome of H. mustelae determined by LC-MS

From: Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

Rank^a	Locus	Annotation	Mw Da	MOWSE Score^b	Coverage^c%	emPAIe^d	Mol%
1	HMU14370	cft ferritin	19,056	455	53	2.72	5.74
2	HMU03320	ahpC alkyl hydroperoxide reductase	22,088	806	68	2.48	5.22
3	HMU12840	aroQ 3-dehydroquinate dehydratase	17,477	105	21	2.16	4.55
4	HMU14250	undefined product; COG4969, tfp pilus assembly protein, major pilin PilA [cell motility and secretion/Intracellular trafficking and secretion]	15,880	241	50	1.42	3
5	HMU00320	undefined product; no putative conserved domains detected, hypothetical protein Lreu23DRAFT_1924 [L. reuteri 100-23]: 35% ID	8,942	158	33	1.37	2.89
6	HMU14210	fldA flavodoxin 1	18,147	231	25	1.31	2.76
7	HMU03050	ureA fusion of urease beta and gamma subunits	25,199	683	60	1.15	2.43
8	HMU03060	ureB urease alpha subunit	61,146	1,218	37	1.048	2.21
9	HMU04000	groEL 60 kD chaperonin (cpn60)	57,443	1,714	51	1.035	2.18
10	HMU12690	possible bacterioferritin	17,198	380	32	1.015	2.14
11	HMU03500	putative amino-acid transporter periplasmic solute-binding protein	30,533	626	52	0.96	2.02
12	HMU01210	tpx probable thiol peroxidase	17,993	350	38	0.83	1.75
13	HMU00630	putative autotransporter protein	151,727	1,371	25	0.82	1.73
14	HMU13930	putative exported protein; COG1464, NlpA, ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism]	28,862	425	30	0.82	1.73
15	HMU01180	putative membrane-anchored cell surface protein	281,808	2,432	22	0.81	1.72
16	HMU03120	tuf elongation factor TU	43,570	850	48	0.77	1.64
17	HMU04030	petA putative putative ubiquinol-cytochrome C reductase iron-sulfur subunit	17,916	227	46	0.77	1.64
18	HMU06410	undefined product; No putative conserved domains detected, hypothetical protein Hac_1480 [Helicobacter acinonychis str. Sheeba]: 29% ID	20,229	254	18	0.77	1.64
19	HMU03990	groES 10 kD chaperonin (cpn10)	10,185	157	31	0.71	1.51
20	HMU09770	trxA thioredoxin	11,553	194	28	0.71	1.51
21	HMU08630	putative outer membrane autotransporter	155,228	968	14	0.64	1.37
22	HMU05030	putative hydantoinase A	78,444	785	30	0.54	1.15
23	HMU13940	putative exported protein; COG1464: ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism]	29,374	430	29	0.54	1.15
24	HMU05020	undefined product; COG4647/pfam08882, acetone_carb_G	14,279	213	29	0.49	1.04
25	HMU01190	putative hypothetical glycine-rich autotransporter protein	194,616	1,281	19	0.48	1.02
26	HMU00600	putative LPXTG surface protein	133,006	651	14	0.47	1
27	HMU09610	secG putative protein-export membrane protein	12,734	81	21	0.46	0.98
28	HMU12860	sodB superoxide dismutase (Fe)	24,576	231	23	0.46	0.98
29	HMU14090	putative thioredoxin	11,796	138	30	0.46	0.98
30	HMU11370	undefined product; no putative conserved domains detected, hypothetical protein Abu_2077 [Arcobacter butzleri RM4018]: 37%	7,677	92	26	0.46	0.98
31	HMU05010	putative hydantoin hydantoinase A	70,769	835	31	0.45	0.96
32	HMU10410	rpsH 30S ribosomal protein S8	14,807	247	32	0.43	0.91
33	HMU10080	putative putative membrane protein	10,902	103	17	0.42	0.89
34	HMU04360	atpC ATP synthase F1 sector epsilon subunit	13,211	108	16	0.42	0.89
35	HMU04350	atpD ATP synthase F1 sector beta subunit	51,310	581	23	0.42	0.89
36	HMU10380	rpsE 30S ribosomal protein S5	15,592	199	33	0.42	0.89
37	HMU09160	undefined product; no putative conserved hits detected, hypothetical protein HH1743 [Helicobacter hepaticus ATCC 51449]: 32% ID	43,936	335	14	0.40	0.85
38	HMU10290	rpoA DNA-directed RNA polymerase alpha chain	37,551	320	24	0.38	0.82
39	HMU05040	putative hydantoin utilization protein B	83,272	514	23	0.37	0.8
40	HMU05840	putative flagellin	53,982	501	27	0.37	0.79
41	HMU10120	peb4[1]f2 major antigenic peptide PEB3ll binding factor 2	31,816	371	32	0.35	0.76
42	HMU04050	petC putative ubiquinol-cytochrome C reductase cytochrome C subunit	33,425	299	25	0.35	0.74
43	HMU04380	exbD3 exbD olR family transport protein	14,662	132	23	0.35	0.74
44	HMU06080	hypothetical protein Cj0372; COG0754/pfarm03738: Glutathionylspermidine synthase [amino acid transport and metabolism]	44,843	197	12	0.35	0.74
45	HMU11040	fliL possible flagellar protein	18,741	175	18	0.33	0.7
46	HMU04330	atpA ATP synthase F1 sector alpha subunit	55,012	371	20	0.31	0.67
47	HMU07380	hupB DNA-binding protein HU homolog	10,122	142	26	0.29	0.61
48	HMU03350	rplS 50S ribosomal protein L19	13,546	226	28	0.29	0.61
49	HMU03180	rplL 50S ribosomal protein L7/L12	12,946	97	18	0.27	0.58
50	HMU01920	htrA serine protease (protease DO)	45,914	305	17	0.27	0.58

The 50 most abundant proteins are tabulated.
a. Relative abundance ranked by mol%
b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence
c. Proportion of each protein sequence identified
d. Exponentially modified protein abundance index. See Methods for details and reference.

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ISSN: 1471-2164

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