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Table 4 The envelope proteome of H. mustelae determined by LC-MS

From: Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen

Ranka Locus Annotation Mw Da MOWSE Scoreb Coveragec% emPAIed Mol%
1 HMU14370 cft ferritin 19,056 455 53 2.72 5.74
2 HMU03320 ahpC alkyl hydroperoxide reductase 22,088 806 68 2.48 5.22
3 HMU12840 aroQ 3-dehydroquinate dehydratase 17,477 105 21 2.16 4.55
4 HMU14250 undefined product; COG4969, tfp pilus assembly protein, major pilin PilA [cell motility and secretion/Intracellular trafficking and secretion] 15,880 241 50 1.42 3
5 HMU00320 undefined product; no putative conserved domains detected, hypothetical protein Lreu23DRAFT_1924 [L. reuteri 100-23]: 35% ID 8,942 158 33 1.37 2.89
6 HMU14210 fldA flavodoxin 1 18,147 231 25 1.31 2.76
7 HMU03050 ureA fusion of urease beta and gamma subunits 25,199 683 60 1.15 2.43
8 HMU03060 ureB urease alpha subunit 61,146 1,218 37 1.048 2.21
9 HMU04000 groEL 60 kD chaperonin (cpn60) 57,443 1,714 51 1.035 2.18
10 HMU12690 possible bacterioferritin 17,198 380 32 1.015 2.14
11 HMU03500 putative amino-acid transporter periplasmic solute-binding protein 30,533 626 52 0.96 2.02
12 HMU01210 tpx probable thiol peroxidase 17,993 350 38 0.83 1.75
13 HMU00630 putative autotransporter protein 151,727 1,371 25 0.82 1.73
14 HMU13930 putative exported protein; COG1464, NlpA, ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism] 28,862 425 30 0.82 1.73
15 HMU01180 putative membrane-anchored cell surface protein 281,808 2,432 22 0.81 1.72
16 HMU03120 tuf elongation factor TU 43,570 850 48 0.77 1.64
17 HMU04030 petA putative putative ubiquinol-cytochrome C reductase iron-sulfur subunit 17,916 227 46 0.77 1.64
18 HMU06410 undefined product; No putative conserved domains detected, hypothetical protein Hac_1480 [Helicobacter acinonychis str. Sheeba]: 29% ID 20,229 254 18 0.77 1.64
19 HMU03990 groES 10 kD chaperonin (cpn10) 10,185 157 31 0.71 1.51
20 HMU09770 trxA thioredoxin 11,553 194 28 0.71 1.51
21 HMU08630 putative outer membrane autotransporter 155,228 968 14 0.64 1.37
22 HMU05030 putative hydantoinase A 78,444 785 30 0.54 1.15
23 HMU13940 putative exported protein; COG1464: ABC-type metal ion transport system, periplasmic component/surface antigen [inorganic ion transport and metabolism] 29,374 430 29 0.54 1.15
24 HMU05020 undefined product; COG4647/pfam08882, acetone_carb_G 14,279 213 29 0.49 1.04
25 HMU01190 putative hypothetical glycine-rich autotransporter protein 194,616 1,281 19 0.48 1.02
26 HMU00600 putative LPXTG surface protein 133,006 651 14 0.47 1
27 HMU09610 secG putative protein-export membrane protein 12,734 81 21 0.46 0.98
28 HMU12860 sodB superoxide dismutase (Fe) 24,576 231 23 0.46 0.98
29 HMU14090 putative thioredoxin 11,796 138 30 0.46 0.98
30 HMU11370 undefined product; no putative conserved domains detected, hypothetical protein Abu_2077 [Arcobacter butzleri RM4018]: 37% 7,677 92 26 0.46 0.98
31 HMU05010 putative hydantoin hydantoinase A 70,769 835 31 0.45 0.96
32 HMU10410 rpsH 30S ribosomal protein S8 14,807 247 32 0.43 0.91
33 HMU10080 putative putative membrane protein 10,902 103 17 0.42 0.89
34 HMU04360 atpC ATP synthase F1 sector epsilon subunit 13,211 108 16 0.42 0.89
35 HMU04350 atpD ATP synthase F1 sector beta subunit 51,310 581 23 0.42 0.89
36 HMU10380 rpsE 30S ribosomal protein S5 15,592 199 33 0.42 0.89
37 HMU09160 undefined product; no putative conserved hits detected, hypothetical protein HH1743 [Helicobacter hepaticus ATCC 51449]: 32% ID 43,936 335 14 0.40 0.85
38 HMU10290 rpoA DNA-directed RNA polymerase alpha chain 37,551 320 24 0.38 0.82
39 HMU05040 putative hydantoin utilization protein B 83,272 514 23 0.37 0.8
40 HMU05840 putative flagellin 53,982 501 27 0.37 0.79
41 HMU10120 peb4[1]f2 major antigenic peptide PEB3ll binding factor 2 31,816 371 32 0.35 0.76
42 HMU04050 petC putative ubiquinol-cytochrome C reductase cytochrome C subunit 33,425 299 25 0.35 0.74
43 HMU04380 exbD3 exbD olR family transport protein 14,662 132 23 0.35 0.74
44 HMU06080 hypothetical protein Cj0372; COG0754/pfarm03738: Glutathionylspermidine synthase [amino acid transport and metabolism] 44,843 197 12 0.35 0.74
45 HMU11040 fliL possible flagellar protein 18,741 175 18 0.33 0.7
46 HMU04330 atpA ATP synthase F1 sector alpha subunit 55,012 371 20 0.31 0.67
47 HMU07380 hupB DNA-binding protein HU homolog 10,122 142 26 0.29 0.61
48 HMU03350 rplS 50S ribosomal protein L19 13,546 226 28 0.29 0.61
49 HMU03180 rplL 50S ribosomal protein L7/L12 12,946 97 18 0.27 0.58
50 HMU01920 htrA serine protease (protease DO) 45,914 305 17 0.27 0.58
  1. The 50 most abundant proteins are tabulated.
  2. a. Relative abundance ranked by mol%
  3. b. Score for the entire protein derived by MASCOT, and made up of the individual scores given to each peptide sequence
  4. c. Proportion of each protein sequence identified
  5. d. Exponentially modified protein abundance index. See Methods for details and reference.